Equipment Available in the LMCF
Nikon A1 Confocal Microscope
- Lasers: 450/50, 525/50, and 600/50-685/70
- Mercury Lamp for Wide-field
- Objectives: 20X, 40X, 60X
- Heated stage for short-term live-cell imaging
- A stand-alone CO2 incubator is available for maintaining cells prior to imaging
Zeiss Axio A1 Upright Fluorescence Microscope
- 100 W mercury lamp
- Filters sets: DAPI, FITC, and Rhodamine
- Objectives: 10X, 63X, and 100X
- Axiocam MRc5 digital camera
Nikon Eclipse TE-300 Inverted Fluorescence Microscope
- Mercury Lamp
- Filter Sets: FITC, DAPI, and Rhodamine
- Objectives: 10X, 20X, 40X, and 100X
- Hoffman optics
- Spot Insight Firewire digital camera
Nikon Labaphot Compound Fluorescence Microscope
- Mercury lamp
- Filter Sets: FITC and DAPI
- Phase contrast optics
- Objectives: 10X, 20X, 40X, and 100X objectives
- Sony progressive scan color analog camera
- Scion Image software including CG-7 Scion frame grabber
Nikon C-12 SMZ1500 Dissecting Fluorescence Microscope
- Mercury lamp
- GFP filter set
- Magnification range: 7.5X-112.5X.
JEOL 1010 Transmission Electron Microscope
- Tungsten hairpin electron source
- 40, 60, 80, and 100kV accelerating voltages
- Bright field, dark field, and SAD imaging modes
- Magnification range of 500X-200,000X
- Images are stored on 3.25鈥� x 4鈥� sheet film or digitized
Motic Compound Microscope
- Fluorescence (LED) with three filter cubes: DAPI, FITC, and Rhodamine.
- SPOT RT image software (v. 4.6)
- SPOT-RT monochrome firewire camera.
Other relevant microscopes: Microscopes presently available at 成人抖阴 include several inverted microscopes used for tissue culture purposes and stereomicroscopes used for imaging whole mount specimens. In addition, a variety of compound microscopes are available to Core users. In each teaching laboratory we also have a variety of Zeiss, Leica, and Nikon compound microscopes and dissecting scopes for student use.
Leica CM 1850 Cryostat
- Chamber temperature down to 鈥�35 掳C
- Actively cooled quick freeze shelf with Peltier unit for freezing down to 鈥�60 掳C.
- Motorized coarse feed at two speeds
- Smooth specimen orientation with automatic
Thermo Microm HM 430 Microtome
- Use for paraffin and frozen sections in biological, botanical, and materials science applications
- Manual or automatic advance
- Specimen fine orientation on X/Y-axes
- Sectioning Range 1 to 60碌m
- Specimen Size 80 x 60mm